Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human & Mouse LC3B II Detection Kit, 10,000 Assay Points
HTRF Human & Mouse LC3B II Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Phospho-c-RAF (Ser43) Detection Kit, 10,000 Assay Points
HTRF Phospho-c-RAF (Ser43) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc Tag-lite pSNAP-HTR4 Serotonin Receptor Plasmid, 10 µg
Tag-lite pSNAP-HTR4 Serotonin Receptor Plasmid, 10 µg
Revvity Health Sciences Inc HTRF Human & Mouse Total NRF2 Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Total NRF2 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human XIAP / BIR3 Binding Kit, 500 Assay Points
HTRF Human XIAP / BIR3 Binding Kit, 500 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human Total HSP27 Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Human Total HSP27 Detection Kit, 50,000 Assay Points
Molecular Devices Fluorescence Filter Slide, 485/20 nm, 525/30 nm
Molecular Devices fluorescence filter slide provides narrow bandpass excitation and emission filters for fluorescence detection in compatible microplate readers. The slide features a 485/20 nm excitation filter and a 525/30 nm emission filter to enable sensitive measurement of visible-range fluorophores.
- Excitation bandpass 485/20 nm.
- Emission bandpass 525/30 nm.
- Designed for fluorescence detection in microplate readers.
- Serves as a replaceable filter slide for reader configurations.
- Supports common visible-range fluorophores for assay flexibility.
Molecular Devices Emission Filter, 485 nm center, 20 nm bandwidth, FilterMax Compatible
Emission filter for fluorescence detection centered at 485 nm with a 20 nm bandwidth. Designed for installation in microplate reader filter slides to isolate emission wavelengths and improve signal specificity in fluorescence assays.
- Emission peak at 485 nm.
- Bandwidth of 20 nm full width.
- Fluorescence emission filter for use in filter slides.
- Compatible with microplate reader fluorescence intensity and polarization measurements.
- Helps reduce spectral overlap and improve assay signal-to-noise ratio.
Molecular Devices Fluorescence Filter, 465 nm, 35 nm, With Holder
Molecular Devices fluorescence band-pass filter supplied with a holder, designed to provide selective optical filtering for fluorescence assays. The filter has a center wavelength of 465 nm and a nominal full width of 35 nm, enabling targeted detection of blue/green fluorophores in multi-mode microplate reader applications.
- Provides a center wavelength of 465 nm for blue/green fluorophore detection.
- 35 nm full width for controlled spectral selection and reduced cross-talk.
- Includes a holder for secure mounting in the instrument filter tray or slide slot.
- Intended for fluorescence detection in multi-mode microplate assays.
- Helps reduce background and improve signal specificity in fluorescence measurements.
Revvity Health Sciences Inc HTRF Human IL-12/IL-23 p40 Detection Kit, 500 Assay Points
HTRF Human IL-12/IL-23 p40 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Total PINK3 Detection Kit, 50,000 Assay Points
HTRF Human Total PINK3 Detection Kit, 50,000 Assay Points
Molecular Devices Filter Lens, 630 nm, Emission
The 630 nm filter lens is an interchangeable emission filter designed to enable red-region fluorescence detection on compatible microplate readers. It provides selective transmission centered at 630 nm to improve signal specificity for assays using red-emitting fluorophores.
- Center wavelength 630 nm.
- Designed for emission detection in the red spectral region.
- Interchangeable filter format for compatible microplate readers.
- Enhances signal specificity and reduces background for fluorescence assays.
- Single-unit form factor suitable for routine assay configuration changes.
Molecular Devices Fluorescence Polarization Filter, 465 nm, 35 nm, Vertical
The Molecular Devices fluorescence polarization filter passes light centered at 465 nm with a 35 nm bandwidth and is polarized for a vertical orientation. Supplied mounted in a holder, the filter is intended as a spare/accessory for compatible multi-mode microplate readers and is optimized for fluorescence polarization assays.
- Passes light centered at 465 nm with a 35 nm bandwidth.
- Vertical polarization to support fluorescence polarization measurements.
- Supplied mounted in a holder for easy insertion into filter slides.
- Optimized for fluorescence polarization assays to improve measurement specificity.
- Reduces background by rejecting off-band light to improve signal-to-noise ratio.
- Handle by the tab only to avoid fingerprints and preserve optical performance.
Revvity Health Sciences Inc HTRF Human Phospho-CHK-2 (Thr68) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-CHK-2 (Thr68) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total BRD9 Detection Kit, 500 Assay Points
HTRF Human & Mouse Total BRD9 Detection Kit, 500 Assay Points